Myc-Tag (19C2) Mouse mAb | AbTract™
Cat No. Quantity Price Availability
M20002-100 100 µl $199 Ship next business day
M20002-200 200 µl $379 Ship next business day
  • Antibody
    • Myc-Tag (19C2) Mouse mAb
  • Isotype
    • IgG1
  • Source
    • This monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
  • Specifity
    • Myc-Tag (19C2) Mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.
  • Storage
    • Store at -20°C. Stable for one year from the date of shipment.
  • Important
    • Use an anti-MOUSE secondary antibody to detect the 19C2 antibody.
  • Recommended Antibody Dilutions
    • Western blotting 1:5000
    • Immunofluorescence 1:2000
    • Immunoprecipitation 1:200
    • ELISA 1:2000
  • Reactivity
    • All

Application Data


Western Blot

  • wb
  • Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 μg of cell lysate.

Immunofluorescence

  • if
  • IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (right, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution.

Immunoprecipitation

  • ip
  • IP of extracts from 293T cells transfected (lane 1) with a Myc-tagged protein and a mock transfection (lane 2, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:200 (1), 1:500 (2), 1:200 (3).