His-Tag (2A8) Mouse mAb | AbTract™
Cat No. Quantity Price Availability
M20001-100 100 µl $199 Ship next business day
M20001-200 200 µl $379 Ship next business day
  • Antibody
    • His-Tag (2A8) Mouse mAb
  • Isotype
    • Mouse IgG1
  • Source
    • This monoclonal antibody is produced by immunizing mice with a 6× His synthetic peptide (KLH-coupled).
  • Specifity
    • His-Tag (2A8) Mouse mAb detects over-expressed or recombinant proteins containing the 6× His epitope tag.
  • Storage
    • Store at -20°C. Stable for one year from the date of shipment.
  • Important
    • Use an anti-MOUSE secondary antibody to detect the 2A8 antibody.
  • Recommended Antibody Dilutions
    • Western blotting 1:5000
    • Immunofluorescence 1:2000
    • Immunoprecipitation 1:100
    • ELISA 1:2000
  • Reactivity
    • All

Application Data


Western Blot

  • wb
  • Western blot analysis of over-expressed His-tagged protein in 293T cell lysate, using Abmart His-tag (2A8) Mouse mAb. The antibody dilutions are1:2000 (lane 1), 1:5000 (lane 2) and 1:10000 (lane 3). Each lane was loaded with 10 μg of cell lysate.
  • wb
  • 2A8 can specifically detect 1ng of recombinant protein. There is 6 ng, 3 ng, 1 ng, and 0 ng His-tagged recombinant protein in lanes 1-4, respectively, added with 20 μg of 293T cell lysate. The 2A8 antibody dilution is 1:2000.

Immunofluorescence

  • if
  • IF analysis of 293T cells transfected (left) with a His-tagged protein and a mock transfection (right, using the same protein without the His tag), using Abmart His-Tag (2A8) Mouse mAb at a 1:2000 dilution.

Immunoprecipitation

  • ip
  • IP of extracts from 293T cells transfected (lane 1) with a His-tagged protein and a mock transfection (right, using the same protein without the His tag), using Abmart His-Tag (2A8) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:100 (1), 1:100 (2).