Epitope Tag Antibodies
HA-Tag (26D11) Mouse mAb | AbTract™
| Cat No. | Quantity | Price | Availability |
| M20003-100 | 100 µl | $199 | Ship next business day |
| M20003-200 | 200 µl | $379 | Ship next business day |
- Antibody
- HA-Tag (26D11) Mouse mAb
- Isotype
- IgG1
- Source
- This monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the infuenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).
- Specifity
- HA-Tag (26D11) Mouse mAb detects over-expressed proteins containing the HA epitope tag.
- Storage
- Store at -20°C. Stable for one year from the date of shipment.
- Important
- Use an anti-MOUSE secondary antibody to detect the 26D11 antibody.
- Recommended Antibody Dilutions
- Western blotting 1:5000
- Immunofluorescence 1:1000
- Immunoprecipitation 1:100
- ELISA 1:2000
- Reactivity
- All
Application Data
Western Blot

- Western blot analysis of 293T cells transfected (lane 1) with a HA-tagged protein and a mock transfection (lane 2, using the same protein without the HA tag), using HA-Tag (26D11) Mouse mAb at a 1:5000 dilution. Each lane was loaded with 10 μg of cell lysate.
Immunofluorescence

- IF analysis of 293T cells transfected with a HA-tagged protein, using HA-Tag (26D11) Mouse mAb at a 1:1000 dilution.
Immunoprecipitation

- IP of extracts from 293T cells transfected (lane 1) with a HA-tagged protein and a mock transfection (lane 2, using the same protein without the HA tag), using HA-Tag (26D11) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:100 (1), 1:100 (2).

