GFP-Tag (7G9) Mouse mAb | aBTract™
Cat No. Quantity Price Availability
M20004-100 100 µl $199 Ship next business day
M20004-200 200 µl $379 Ship next business day
  • Antibody
    • GFP-Tag (7G9) Mouse mAb
  • Isotype
    • IgG1
  • Source
    • This monoclonal antibody is produced by immunizing animals with full-length recombinant GFP.
  • Specifity
    • GFP-Tag (7G9) Mouse mAb detects GFP and GFP fusion proteins.
  • Storage
    • Store at -20°C. Stable for one year from the date of shipment.
  • Important
    • Use an anti-MOUSE secondary antibody to detect the 7G9 antibody.
  • Recommended Antibody Dilutions
    • Western blotting 1:5000
    • Immunofluorescence 1:500
    • Immunoprecipitation 1:50
    • ELISA 1:2000
  • Reactivity
    • All

Application Data


Western Blot

  • wb
  • Western blot analysis of over-expressed GFP-tagged protein in 293T cell lysate, using GFP-tag (7G9) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 μg of cell lysate.

Immunofluorescence

  • if
  • IF analysis of 293T cells transfected with a GFP-tagged protein, using GFP-Tag (7G9) Mouse mAb at a 1:500 dilution.

Immunoprecipitation

  • ip
  • IP of extracts from 293T cells transfected (lane 1,2) with a GFP-tagged protein and a mock transfection (lane 3, using the same protein without the GFP tag), using GFP-Tag (7G9) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:50 (1), 1:100 (2), 1:50 (3).