GFP Antibody | AbTract™
Cat No. Quantity Price Availability
P30010-100 100 µl $199 Ship next business day
  • Antibody
    • GFP Antibody
  • Isotype
    • Rabbit IgG
  • Source
    • This polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of EGFP.
  • Specifity
    • GFP Antibody detects GFP and GFP fusion proteins.
  • Storage
    • Store at -20°C. Stable for one year from the date of shipment.
  • Important
    • Use an anti-RABBIT secondary antibody to detect the GFP Antibody.
  • Recommended Antibody Dilutions
    • Western blotting 1:2000
    • Immunoprecipitation 1:100
  • Reactivity
    • All

Application Data


Western Blot

  • wb
  • Western blot analysis of over-expressed GFP protein in COS7 cell lysate, using GFP rabbit polyclonal antibody. The antibody dilutions are 1:1000 (lane1), 1:2000 (lane2), 1:5000 (lane3), 1:10000 (lane4). Each lane was loaded with 10 µg of cell lysate.

Immunoprecipitation

  • ip
  • IP of extracts from COS7 cells transfected with a GFP-tagged protein using GFP rabbit polyclonal antibody (5 µl, lane 3) or using normal rabbit IgG (lane 2) as control, followed by Western blot using GFP (7G9) Mouse mAb (dilution: 1:5000). Lane 1 was loaded with 10 µg of COS7 cell lysate over-expressing a GFP-tagged protein.