DYKDDDDK-Tag (3B9) Mouse mAb | AbTract™
Cat No. Quantity Price Availability
M20008-100 100 µl $199 Ship next business day
M20008-200 200 µl $379 Ship next business day
  • Antibody
    • DYKDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma’s Anti-FLAG® M2 Antibody)
  • Isotype
    • Mouse IgG2b
  • Source
    • This monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).
  • Specifity
    • DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.
  • Storage
    • Store at -20°C. Stable for one year from the date of shipment.
  • Important
    • Use an anti-MOUSE secondary antibody to detect the 3B9 antibody.
  • Recommended Antibody Dilutions
    • Western blotting 1:5000
    • Immunofluorescence 1:1000
    • Immunoprecipitation 1:100
    • ELISA 1:2000
  • Reactivity
    • All

Application Data


Western Blot

  • wb
  • Western blot analysis of over-expressed DYKDDDDK-tagged protein in 293T cell lysate, using DYKDDDDK-Tag (3B9) Mouse mAb. The antibody dilutions are 1:2000 (lane 1), 1:5000 (lane 2), 1:10000 (lane 3) and 1:20000 (lane 4). Each lane was loaded with 2 μg of cell lysate. Lane 5 is 2 μg of mock transfected cell lysate at a dilution of 1:5000.

Immunofluorescence

  • if
  • IF analysis of 293T cells transfected with a DYKDDDDK-tagged protein, using DYKDDDDK-Tag (3B9) Mouse mAb at a 1:1000 dilution.

Immunoprecipitation

  • ip
  • Lane 1: 293T cell lysate transfected with DYKDDDDK-tag protein. Lane 2: Mock transfected 293T cell lysate. The antibody dilution is 1:100.