Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) | AbTract™
Cat No. Quantity Price Availability
M20018-500 500 µl $358 Ship next business day
  • Antibody
    • Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma’s Anti-FLAG® M2 Antibody)
  • Description
    • Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) is a monoclonal anti-DYKDDDDK antibody covalently linked to agarose; the agarose enables immunoprecipitation (IP) of DYKDDDDK tagged proteins or co-immunoprecipitation (Co-IP) of their interacting partners.
  • Isotype
    • Mouse IgG2b
  • Source
    • This monoclonal antibody is produced by immunizing animals with a synthetic peptide containing epitope DYKDDDDK (KLH-coupled).
  • Specifity
    • Anti-DYKDDDDK-Tag Mouse mAb detects transfected proteins containing the DYKDDDDK epitope tag.
  • Storage
    • The product is supplied as a 50% slurry in storage buffer (1× PBS, pH 7.4, containing 0.1% NaN3). Store the product at 4°C and do not freeze.
  • Reactivity
    • All

Application Data


Immunoprecipitation

  • ip
  • HEK 293T cells were transfected with DYKDDDDK-tagged protein or not, and 100 μl cell lysate (about 100 μg total protein) was incubated with 30 μl 50% slurry of Anti-DYKDDDDK Agarose for 3 h at 4°C. After washing, the beads were eluted by 60 μl elution buffer. After neutralization of the eluant, 12 μl 6× SDS loading buffer was added. Then 20 μl sample was subjected to the SDS-PAGE. Blot was probed with Anti-DYKDDDDK-Tag Mouse mAb.
    Lane 1: Total cell lysate of transfected HEK 293T cells.
    Lane 2: Elution with elution buffer.
    Lane 3: IP of untransfected HEK 293T lysate.