Streamline your workflow today with our SEAL™ derived guaranteed mAbs and pAbs. With the application of the SEAL™ platform all potential protein surface epitopes are selected based on proprietary antigen design algorithms. The epitopes are biologically converted into potent immunogens using a proprietary immunogenicity-enhancement system. This is then followed by a proprietary short-duration immunization procedure producing high level of IgG immune responses. A high-throughput hybridoma process is employed to isolate hundreds of high affinity, monoclonal, stable clones.


Guaranteed Antibody Solutions. When you require specialized solutions to meet your research objectives count on our custom antibody services to deliver.


Endogenous WB guaranteed monoclonal antibody
Item Description
Package Name Endogenous WB guaranteed monoclonal antibody
Package code BC-M0010
Pricing USD $5400
Service Guarantee BioChemia Standards: at least one clone specifically detects the overexpressed protein in WB.
Client Standards: at least one clone works in endogenous WB assay
Customer to Provide 1. Name, species, sequence, and NCBI Number for target protein;
2. Full length cDNA plasmid
Service Contents 1. Antigen design and production
2. Immunize 3 Balb/c mice
3. Fusion and ELISA screening
4. Subclone 2-4 rounds
5. Antibody production
6. Protein G affinity purification
7. Antibody validation
Data to Provide For each cell line:
1. Comprehensive validation data on overexpressed protein, including ELISA, WB, IP and IF data.
2. Epitope and affinity data (if available)
3. ELISA and dot blot data on immunogen
Deliverables First Shipment: any samples of the client's interest (50 µl each)
Second Shipment: one hybridoma cell line with 2 mg purified antibody
Timeline 4 months from antigen synthesis to sample delivery
Charge and Refund
1. Client shall pay the prepayment before project initiation; the prepayment will be refunded if BioChemia fails to meet the BioChemia Standards.
2. Client shall pay the final payment when Client confirms a qualified clone meeting Client Standards
*Terms & Conditions: Gifts valid for antibody orders made in a single purchase order above a value of $2,000 (USD/CAN), excluding freight, taxes and custom duties, starting June 1st, 2011 to December 31th, 2011. The promotion is avaliable to eligible customers in the US, Canada, UK, EU countries and Israel. Orders can be divided for multiple iTouchs at a minimum purchase value equaling or greater than $2,000 (USD/CAN). Promotion not available for tenders, bulk purchase with discount. Please check with your institution for eligibility to receive gift items. Apple is not a sponsor of this promotion. iTouch is a trademark of Apple Inc. Other restrictions may apply. BioChemia Inc. reserves the right to substitute and/or terminate this promotion without prior notice.